Biochemistry Laboratory Manual For Undergraduates : (Record no. 535117)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04543nam a22006015i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 9783110411331 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-B1597 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200803184527.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS--GENERAL INFORMATION | |
| fixed length control field | m|||||o||d|||||||| |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr || |||||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 190615s2014 pl fo d z eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9783110411331 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.2478/9783110411331 |
| Source of number or code | doi |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (DE-B1597)445659 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)1013967206 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)954878217 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | DE-B1597 |
| Language of cataloging | eng |
| Transcribing agency | DE-B1597 |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 044 ## - COUNTRY OF PUBLISHING/PRODUCING ENTITY CODE | |
| MARC country code | pl |
| ISO country code | PL |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QD415.5 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI007000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI010000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI013000 |
| Source | bisacsh |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Field link and sequence number | 1p |
| Classification number | 570 |
| -- | DE-101 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Gerczei Fernandez, Timea, |
| Relator term | author. |
| 245 10 - TITLE STATEMENT | |
| Title | Biochemistry Laboratory Manual For Undergraduates : |
| Remainder of title | An Inquiry-Based Approach / |
| Statement of responsibility, etc | Timea Gerczei Fernandez, Scott Pattison. |
| 264 #1 - Production, Publication, Distribution, Manufacture, and Copyright Notice (R) | |
| Place of production, publication, distribution, manufacture | Warsaw ; |
| -- | Berlin : |
| Name of producer, publisher, distributor, manufacturer | De Gruyter Open Poland, |
| Date of production, publication, distribution, manufacture, or copyright notice | [2014] |
| 264 #4 - Production, Publication, Distribution, Manufacture, and Copyright Notice (R) | |
| Date of production, publication, distribution, manufacture, or copyright notice | ©2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource |
| 336 ## - Content Type (R) | |
| Content type term (R) | text |
| Content type code (R) | txt |
| Source (NR) | rdacontent |
| 337 ## - Media Type (R) | |
| Media type term (R) | computer |
| Media type code (R) | c |
| Source (NR) | rdamedia |
| 338 ## - Carrier Type (R) | |
| Carrier type term (R) | online resource |
| Carrier type code (R) | cr |
| Source (NR) | rdacarrier |
| 347 ## - | |
| -- | text file |
| -- | |
| -- | rda |
| 505 00 - FORMATTED CONTENTS NOTE | |
| Title | Frontmatter -- |
| -- | Preface -- |
| -- | Contents -- |
| -- | 1 Introducing the Bacterial Antibiotic Sensor Mini Project -- |
| -- | 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function -- |
| -- | 3 Designing Primers for Site-Directed Mutagenesis -- |
| -- | 4 Performing Site-Directed Mutagenesis -- |
| -- | 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy -- |
| -- | 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease -- |
| -- | 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro -- |
| -- | 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry -- |
| -- | 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching -- |
| -- | 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy -- |
| -- | 11 Understanding the Importance of Buffers in Biological Systems -- |
| -- | 12 Molecular Visualization of an Enzyme, Acetylcholinesterase -- |
| -- | 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment -- |
| -- | 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC -- |
| -- | List of Figures |
| 506 0# - RESTRICTIONS ON ACCESS NOTE | |
| Terms governing access | Open Access |
| Uniform Resource Identifier | <a href="https://purl.org/coar/access_right/c_abf2">https://purl.org/coar/access_right/c_abf2</a> |
| Standardized terminology for access restriction | unrestricted online access |
| Source of term | star |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic. |
| 538 ## - SYSTEM DETAILS NOTE | |
| System details note | Mode of access: Internet via World Wide Web. |
| 540 ## - TERMS GOVERNING USE AND REPRODUCTION NOTE | |
| Terms governing use and reproduction | This eBook is made available Open Access. Unless otherwise specified individually in the content, the work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND) license: |
| Uniform Resource Identifier | <a href="https://creativecommons.org/licenses/by-nc-nd/3.0 ">https://creativecommons.org/licenses/by-nc-nd/3.0 </a> |
| -- | <a href="https://www.degruyter.com/dg/page/open-access-policy">https://www.degruyter.com/dg/page/open-access-policy</a> |
| 546 ## - LANGUAGE NOTE | |
| Language note | In English. |
| 588 0# - | |
| -- | Description based on online resource; title from PDF title page (publisher's Web site, viewed 15. Jun 2019) |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Biochemie |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Biochemistry. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Labortechnik |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| -- | (DE-601)106376314 |
| -- | (DE-588)4006777-4 |
| Topical term or geographic name as entry element | Biochemie |
| Source of heading or term | gnd |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | SCIENCE / Life Sciences / Biochemistry. |
| Source of heading or term | bisacsh |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pattison, Scott, |
| Relator term | author. |
| 776 0# - ADDITIONAL PHYSICAL FORM ENTRY | |
| Qualifying information | EPUB |
| International Standard Book Number | 9783110426250 |
| 776 0# - ADDITIONAL PHYSICAL FORM ENTRY | |
| Qualifying information | |
| International Standard Book Number | 9783110411324 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.2478/9783110411331">https://doi.org/10.2478/9783110411331</a> |
| Public note | Open Access |
| 856 42 - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | Cover |
| Uniform Resource Identifier | <a href="https://www.degruyter.com/cover/covers/9783110411331.jpg">https://www.degruyter.com/cover/covers/9783110411331.jpg</a> |
| 912 ## - | |
| -- | GBV-deGruyter-alles |
| 912 ## - | |
| -- | ZDB-23-GOA |
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