Techniques in quantification and localization of gene expression /
Techniques in quantification and localization of gene expression /
edited by Bruce K. Patterson.
- Birkhäuser, c2000.
- xvii, 150 p., [2] p. of plates : ill. (some col.) ; 24 cm.
Includes bibliographical references and index.
Basic Methods. Basic Flow Cytometry Instrumentation / Fixation for In Situ Molecular Analysis / A Method for Reduction of Green Wavelength Autofluorescence Emission in Cellular Preparations / Gene Quantification: Choosing the Target / Detection and Quantification of Cytokine-Producing Cells by Immunostaining / In Situ Hybridization Using the bDNA Technology / Specific Applications. Kaposi's Sarcoma - Associated Human Herpes Virus Eight: Localization of Viral Gene Expression and Viral Latency / Pathogenetic Mechanisms of Animal Orbiviruses That Cause Disease at Low Copy Number / Charles L. Goolsby and Cathy James. Bruce K. Patterson and Daniel K. Jiyamapa. Victoria L. Mosiman. Bruce K. Patterson. Ulf Andersson, Mark J. Litton and Tom E. Fehniger / [et al.]. Vincent P. Antao, Audrey N. Player and Janice A. Kolberg -- Scott J. Brodie, John N. Krieger and Catherine Diamond. Scott J. Brodie, Patricia M. O'Hearn and Kurt Diem / [et al.]. 1. 2. 3. 4. 5. 6. 7. 8. Localization of Hepatitis C Virus Using Reverse Transcriptase Fluorescence in Situ 5' Nuclease Assay / Simultaneous Quantification and Localization of HIV gag-pol Expression Using Ultrasensitive Fluorescence In Situ Hybridization / Quantification and Localization of Gene Expression: Future Directions / Bruce K. Patterson. Bruce K. Patterson. Bruce K. Patterson. 9. 10. 11.
"Techniques in Quantification and Localization of Gene Expression describes in detail the methods used to quantify and localize gene expression. As an organism's DNA can be considered to be its genetic potential, its RNA and protein expression are the functional result of its genetic program. Localizing gene expression is therefore an important tool in basic cell and molecular biology research and is particularly important in helping to understand the molecular basis of disease.". "Sensitive detection of gene expression is hindered by poor recovery or preservation of target sequences. This book emphasizes methods that are optimized to ensure full recovery and preservation of gene expression targets from specimen acquisition through to detection and final analysis." "Researchers and students in cellular biology will find this book a very useful guide to gene localizing techniques."--BOOK JACKET.
0817640347 3764340347
99027046
Gene expression--Research--Methodology.
ბიოლოგია ბიოქიმია ნუკლეინის მჟავები
QH450 / .T43 2000
572.8/65
577.113
Includes bibliographical references and index.
Basic Methods. Basic Flow Cytometry Instrumentation / Fixation for In Situ Molecular Analysis / A Method for Reduction of Green Wavelength Autofluorescence Emission in Cellular Preparations / Gene Quantification: Choosing the Target / Detection and Quantification of Cytokine-Producing Cells by Immunostaining / In Situ Hybridization Using the bDNA Technology / Specific Applications. Kaposi's Sarcoma - Associated Human Herpes Virus Eight: Localization of Viral Gene Expression and Viral Latency / Pathogenetic Mechanisms of Animal Orbiviruses That Cause Disease at Low Copy Number / Charles L. Goolsby and Cathy James. Bruce K. Patterson and Daniel K. Jiyamapa. Victoria L. Mosiman. Bruce K. Patterson. Ulf Andersson, Mark J. Litton and Tom E. Fehniger / [et al.]. Vincent P. Antao, Audrey N. Player and Janice A. Kolberg -- Scott J. Brodie, John N. Krieger and Catherine Diamond. Scott J. Brodie, Patricia M. O'Hearn and Kurt Diem / [et al.]. 1. 2. 3. 4. 5. 6. 7. 8. Localization of Hepatitis C Virus Using Reverse Transcriptase Fluorescence in Situ 5' Nuclease Assay / Simultaneous Quantification and Localization of HIV gag-pol Expression Using Ultrasensitive Fluorescence In Situ Hybridization / Quantification and Localization of Gene Expression: Future Directions / Bruce K. Patterson. Bruce K. Patterson. Bruce K. Patterson. 9. 10. 11.
"Techniques in Quantification and Localization of Gene Expression describes in detail the methods used to quantify and localize gene expression. As an organism's DNA can be considered to be its genetic potential, its RNA and protein expression are the functional result of its genetic program. Localizing gene expression is therefore an important tool in basic cell and molecular biology research and is particularly important in helping to understand the molecular basis of disease.". "Sensitive detection of gene expression is hindered by poor recovery or preservation of target sequences. This book emphasizes methods that are optimized to ensure full recovery and preservation of gene expression targets from specimen acquisition through to detection and final analysis." "Researchers and students in cellular biology will find this book a very useful guide to gene localizing techniques."--BOOK JACKET.
0817640347 3764340347
99027046
Gene expression--Research--Methodology.
ბიოლოგია ბიოქიმია ნუკლეინის მჟავები
QH450 / .T43 2000
572.8/65
577.113