TY  - BOOK
AU  - McCarthy,Desmond A.
AU  - Macey,Marion G.
TI  - Cytometric analysis of cell phenotype and function
SN  - 9780511526985 (ebook)
AV  - QH585.5.F56 C98 2001
U1  - 571.6/028 21
PY  - 2001///
CY  - Cambridge
PB  - Cambridge University Press
KW  - Flow cytometry
KW  - Handbooks, manuals, etc
KW  - Cytology
N1  - Title from publisher's bibliographic system (viewed on 05 Oct 2015); Principles of flow cytometry / Marion G. Macey -- Introduction to the general principles of sample preparation / Desmond A. McCarthy -- Fluorescence and fluorochromes / Desmond A. McCarthy -- Data analysis in flow cytometry / Mark W. Lowdell -- Laser scanning cytometry: application to the immunophenotyping of hematological malignancies / Richard J. Clatch -- Leukoycte immunobiology / Marion G. Macey -- Immunophenotypic analysis of leukocytes in disease / Jamie D. Cavenagh, Marion G. Macey and Timothy M. Milne -- Analysis and isolation of minor cell populations / Terry Hoy -- Cell cycle, DNA and DNA ploidy analysis / Paul D. Allen and Adrian C. Newland -- Cell viability, necrosis and apoptosis / Paul D. Allen and Adrian C. Newland -- Phagocyte biology and function / Marion G. Macey -- Intracellular measures of singalling pathways / James W. Jacobberger and David W. Hedley -- Cell-cell interactions / Marion G. Macey and Mary R. Cahill -- Nucleic acids / Charles L. Goolsby -- Microbial infections / David Lloyd, David J. Mason and Marc T.E. Suller -- Leucoyte cell surface antigens / Marion G. Macey -- Recent and future developments: conclusions / Desmond A. McCarthy -- Appendix -- Index
N2  - Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings, following improvements in instrument design and computing power and the increased availability of fluorescent agents. This book provides a comprehensive introduction to the theory and clinical applications of these techniques in the assessment of cell phenotype and function. With an emphasis on clinical relevance, the book presents the principles and potential of cytometry in the analysis of phenomena including cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor shedding and apoptosis. Guidance is given on data interpretation, quality control procedures, pitfalls and problems. All chapters contain detailed protocols from leading authorities in the field with extensive practical experience
UR  - https://doi.org/10.1017/CBO9780511526985
ER  -