TY - BOOK AU - Watson,James V. TI - Introduction to flow cytometry SN - 9780511565090 (ebook) AV - QH585.5.F56 W38 1991 U1 - 574.87/028 20 PY - 1991/// CY - Cambridge PB - Cambridge University Press KW - Flow cytometry KW - Diagnostic use KW - Cancer KW - Diagnosis N1 - Title from publisher's bibliographic system (viewed on 05 Oct 2015) N2 - Flow cytometry is a technique for measuring both scattered light and fluorescence from single cells at very rapid rates. Typically up to 5000 cells can be analysed per second. Using various fluorochromes this allows a cell population to be analysed for cells showing certain characteristics such as the presence of a particular enzyme, cellular constituent or other gene product. The information it can provide is invaluable in helping to diagnose certain cancers as well as aiding pure research into many aspects of cell biology such as the cell cycle and gene expression. This book describes the technology in a simple and direct way. The fundamental concepts upon which the technology is based are outlined and the book goes on to describe flow cytometers and what can be gained from using them in biology and medicine. The book gives a particularly detailed account of how artifactual results can arise and where 'noise' is generated. Anyone starting to use, or already using this technique, will need to read this book UR - https://doi.org/10.1017/CBO9780511565090 ER -