000			03442nam a22003857a 4500
001			2450097
003			Ge_NSL
005			20250317085942.0
008			990409s2000 mauaf b 001 0 eng
010			_a 99027046
020			_a0817640347
020			_a3764340347
035			_a(OCoLC)ocm41211439
040			_aDLC _cDLC _dC#P _dOrLoB-B
041			_aeng
042			_apcc
050	0	0	_aQH450 _b.T43 2000
080			_a577.113
082	0	0	_a572.8/65 _221
245	0	0	_aTechniques in quantification and localization of gene expression / _cedited by Bruce K. Patterson.
260			_aBoston : _bBirkhäuser, _cc2000.
300			_axvii, 150 p., [2] p. of plates : _bill. (some col.) ; _c24 cm.
504			_aIncludes bibliographical references and index.
505	0	0	_tBasic Methods. _g1. _tBasic Flow Cytometry Instrumentation / _rCharles L. Goolsby and Cathy James. _g2. _tFixation for In Situ Molecular Analysis / _rBruce K. Patterson and Daniel K. Jiyamapa. _g3. _tA Method for Reduction of Green Wavelength Autofluorescence Emission in Cellular Preparations / _rVictoria L. Mosiman. _g4. _tGene Quantification: Choosing the Target / _rBruce K. Patterson. _g5. _tDetection and Quantification of Cytokine-Producing Cells by Immunostaining / _rUlf Andersson, Mark J. Litton and Tom E. Fehniger / _r[et al.]. _g6. _tIn Situ Hybridization Using the bDNA Technology / _rVincent P. Antao, Audrey N. Player and Janice A. Kolberg -- _tSpecific Applications. _g7. _tKaposi's Sarcoma - Associated Human Herpes Virus Eight: Localization of Viral Gene Expression and Viral Latency / _rScott J. Brodie, John N. Krieger and Catherine Diamond. _g8. _tPathogenetic Mechanisms of Animal Orbiviruses That Cause Disease at Low Copy Number / _rScott J. Brodie, Patricia M. O'Hearn and Kurt Diem / _r[et al.].
505	8	0	_g9. _tLocalization of Hepatitis C Virus Using Reverse Transcriptase Fluorescence in Situ 5' Nuclease Assay / _rBruce K. Patterson. _g10. _tSimultaneous Quantification and Localization of HIV gag-pol Expression Using Ultrasensitive Fluorescence In Situ Hybridization / _rBruce K. Patterson. _g11. _tQuantification and Localization of Gene Expression: Future Directions / _rBruce K. Patterson.
520	1		_a"Techniques in Quantification and Localization of Gene Expression describes in detail the methods used to quantify and localize gene expression. As an organism's DNA can be considered to be its genetic potential, its RNA and protein expression are the functional result of its genetic program. Localizing gene expression is therefore an important tool in basic cell and molecular biology research and is particularly important in helping to understand the molecular basis of disease.".
520	8		_a"Sensitive detection of gene expression is hindered by poor recovery or preservation of target sequences. This book emphasizes methods that are optimized to ensure full recovery and preservation of gene expression targets from specimen acquisition through to detection and final analysis." "Researchers and students in cellular biology will find this book a very useful guide to gene localizing techniques."--BOOK JACKET.
650		0	_aGene expression _xResearch _xMethodology. _95554
653			_aბიოლოგია
653			_aბიოქიმია
653			_aნუკლეინის მჟავები
700			_aPatterson, Bruce K. _eრედაქტორი _95555
900			_bTOC
942			_2udc _cBK
999			_c2139 _d2139