000			03087nam a22003378i 4500
001			CR9780511626531
003			UkCbUP
005			20200124160312.0
006			m|||||o||d||||||||
007			cr||||||||||||
008			090916s2009||||enk o ||1 0|eng|d
020			_a9780511626531 (ebook)
020			_z9780521884532 (hardback)
020			_z9780521711517 (paperback)
040			_aUkCbUP _beng _erda _cUkCbUP
050	0	0	_aR857.B54 _bL33 2009
082	0	0	_a610.28/4 _222
245	0	0	_aLabel-free biosensors : _btechniques and applications / _cedited by Matthew A. Cooper.
264		1	_aCambridge : _bCambridge University Press, _c2009.
300			_a1 online resource (x, 288 pages) : _bdigital, PDF file(s).
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
500			_aTitle from publisher's bibliographic system (viewed on 05 Oct 2015).
505	0		_aLabel-free optical biosensors : an introduction / Brian T. Cunningham -- Experimental design / Robert Karlsson -- Extracting affinity constants from biosensor binding responses / Rebecca L. Rich and David G. Myszka -- Extracting kinetic rate constants from binding responses / Rebecca L. Rich and David G. Myszka -- Sensor surfaces and receptor deposition / Matthew A. Cooper -- Macromolecular interactions / Francis Markey -- Interactions with membranes and membrane receptors / Matthew A. Cooper -- Application of SPR technology to pharmaceutical relevant drug-receptor interactions / Walter Huber -- High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors / Ye Fang ... [et al.] -- ITC-derived binding constants : using microgram quantities of protein / Richard K. Brown ... [et al.] -- Electrical impedance technology applied to cell-based assays / Ryan P. McGuinness and Edward Verdonk.
520			_aLabel-free biosensors are devices that use biological or chemical receptors to detect analytes (molecules) in a sample. They give detailed information on the selectivity, affinity, and, in many cases, also the binding kinetics and thermodynamics of an interaction. Although they can be powerful tools in the hands of a skilled user, there is often a lack of knowledge of the best methods for using label-free assays to screen for biologically active molecules and accurately and precisely characterize molecular recognition events. This book reviews both established and newer label-free techniques giving both the expert user and the general reader interested in the technologies and applications behind label-free an insight into the field from expert opinion leaders and practitioners of the technologies. Most importantly, chapters contain worked examples from leaders in the field that take the reader through the basics of experimental design, setup, assay development, and data analysis.
650		0	_aBiosensors.
700	1		_aCooper, M. A. _q(Matthew A.), _eeditor.
776	0	8	_iPrint version: _z9780521884532
856	4	0	_uhttps://doi.org/10.1017/CBO9780511626531
999			_c521180 _d521178